Plant & Animal Genome IX Conference

Poster: Sequencing & EST
P01_14.html

FUNCTIONAL GENOMIC ANALYSIS OF POTATO TUBER LIFE-CYCLE

Laboratory of Plant Breeding - Dept. of Plant Sciences - WUR, Lawickse Allee 166, Wageningen, 6708 DB, The Netherlands.

Potato tuber life-cycle is composed of a large number of individual developmental stages including tuber formation, tuber development, dormancy and sprouting. We have used cDNA-AFLP fingerprinting to analyse gene expression in 24 individual stages of development, over the period from stolon formation through sprouting. In addition to these developmental stages, different tissues were analysed to assess tissue specificity and various controls were incorporated to determine process specificity. In total, around 18000 transcript-derived cDNA-fragments (TDFs) were visualised from which circa 2600 were included in a statistical analysis allowing general conclusions about gene expression during development. More than 200 process specific TDFs were isolated and sequenced throughout the potato tuber life-cycle. The sequence similarities of these TDFs to known genes give an insight into the kinds of processes occurring during tuberisation, dormancy and sprouting.