Plant & Animal Genome IX Conference Plant & Animal Genome IX Conference
Poster: Sequencing & EST
P01_09.html
Segregation distortion of alleles was observed in several regions of the genome during allele type analyses of genetic markers for the swine resource family at the National Institute of Animal Industry (NIAI), Japan. The resource family was constructed by crossing Meishan females and G÷ttingen miniature male pig. A genetic region on swine chromosome (SSC) 6q was identified as responsible for the distortion, there by terminating conceptus development. Further analysis indicated that the gene(s) function in an early stage of fetal development after the embryo has been attached to the uterus. In order to obtain candidate genes responsible for the termination of embryo development in the region, we performed massive analysis of ESTs obtained from a cDNA library constructed from a 28-day old normal pig embryo, and mapped them by INRA-University of Minnesota porcine Radiation Hybrid panels (IMpRH). The RH mapping provides a basic tool for pig genomics and the comparative map will facilitate identification of candidate genes for disease and economically important traits. From the more than 2000 ESTs analyzed, 700 showed high homology (>80) with known genes or ESTs in the public DNA databases. We have mapped 64 genes out of the 700 on the IMpRH panel. Eighteen of the genes mapped revealed new regional correspondences of chromosome between swine and human. The new regional correspondences are between SSC3 and HSA1, HSA10; SSC4 and HSA6; SSC5 and HSA2, HSA15, HSA16; SSC6 and HSA3, HSA6, HSA20; SSC7 and HSA11; SSC8 and HSA3, HSA6, HSA7; SSC9 and HSA8; SSC13 and HSA1; SSC14 and HSA13; SSC15 and HAS19; and SSC16 and HSA9. These new loci map and correspondences will provide further density to the comparative map between human and swine. Also the new correspondences detected in this study give further insights into the conservation of synteny between swine and human.