1 Molecular Animal Genetics Centre, CSIRO Tropical Agriculture, Level 3, Gehrmann Laboratories, Research Road, University of Queensland, St. Lucia, QLD 4072, Australia 2 Australian Institute of Marine Science, PMB 3 Townsville Mail Centre, QLD 4810, Australia 3 Dept. of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok,10330, Thailand 4 Dept. Agriculture, Food and Nutritional Science, University of Alberta, Edmonton T6G 2P5, Alberta, Canada
The black tiger shrimp P. monodon is the most important shrimp aquaculture species in South East Asia and Australia. With rapid development of genetic marker technologies and their potential benefits to commercial industry through Marker Assisted Selection, the international collaboration between different parties on genetic mapping of P. monodon was initiated in 1998. Type II markers, predominantly AFLP (Amplified Fragment Length Polymorphism) markers were generated from four full-sib families in order to construct initial linkage maps. Each family consisted of 42 F2 animals. Using 19 AFLP primer combinations, linkage analysis has been performed across these families. Male specific and female specific markers have been treated separately within each family. In order to combine information from different families to make a consensus map, eight microsatellite markers (tri- and tetra nucleotide) are being used to genotype the progeny across all families. The common AFLP markers and microsatellites will provide links to identify the homologous linkage groups between family maps.
Plant & Animal Genome VIII Conference Plant & Animal Genome VIII Conference