1 National Center for Genetic Engineering and Biotechnology at Kasetsart University, Kampangsaen, Nakorn Pathom 73140, Thailand 2 Marine Science Department, Fisheries Faculty, Kasetsart University, Bangkok,Thailand 3 DNA Technology Lab, National Center for Genetic Engineering and Biotechnology at Kasetsart University, Kampangsaen, Nakorn Pathom 73140, Thailand 4 Agronomy Department, Kasetsart University, Kampangsaen, Nakorn Pathom 73140, Thailand
Morphological characters have been used as a tool for identifying shrimp species. However, closely related species are difficult to distinguish morphologically. Development of molecular markers would be useful not only for species identification but also for shrimp breeding. We have been developing more than 100 simple sequence length polymorphism (SSLP) markers from genomic DNA of back tiger shrimp by enrichment with eight biotinylated oligoes, (AG)10, (TG)10, (CAT)10, (GAA)10, (CATA)8, (GATA)8, and (TCAG)8. The trinucleotide repeat (CAT)n, dinucleotide repeat (AG)n and tetranucleotide repeat (TCAG)n was predominantly observed in a portion of 37, 21 and 15% respectively in the enriched libraries. Four PCR-based markers, mitochondria-specific, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and SSLP, were used to compare the classification analysis using twelve shrimp species. RAPD and AFLP markers have a powerful to provide large amount of visualized polymorphism. However, genetic diversity using these markers should be analyzed with large number of primer combinations. Phylogenetic tree based on Mitochondrial-specific of 12s and 16s rDNA or SSLP markers and morphological characters showed similar relationship. Moreover, mitochondrial-specific and SSLP markers are suitable to investigate inter and intra-specific variation in shrimps. Potential use across shrimp species makes SSLP the appropriate tool for genetic studies and breeding in shrimp.
Plant & Animal Genome VIII Conference Plant & Animal Genome VIII Conference