USE OF FLUORESCENT TECHNOLOGY FOR GENOMIC MAPPING IN PINK SALMON

Wild Trout and Salmon Genetics Lab, Division of Biological Sciences, University of Montana, Missoula, MT 59812 USA

We are using fluorescent technology and the Hitachi FMBIO100 to construct a linkage map for the pink salmon Oncorhynchus gorbuscha genome. We are using several techniques to generate a sufficient number of markers to produce this map using a single family. We have analyzed the segregation of 393 Amplified Fragment Length Polymorphisms (AFLP's), 162 Paired Interspersed Nuclear Elements (PINEs), 28 microsatellites, 35 Randomly Amplified Polymorphic DNA fragments (RAPD's), and two Comparative Anchor Tagged Sequences (CATS) in a single female and 94 of her haploid progeny. In addition, we have gene-centromere mapped many of these same loci using gynogenetic diploid offspring of this same female. We can now compare the results of these two mapping systems to confirm that the scoring of markers, most of which are dominant, is reliable. Our current efforts focus on using Single Strand Conformational Polymorphisms (SSCP) and heteroduplex analysis to increase the number of anchor loci included on the map. These loci will allow us to more efficiently incorporate data from other salmonid maps and will facilitate other comparative mapping efforts.