MAPPING OF INTROGRESSED COTTON POPULATIONS WITH DNA MARKERS

P.O. Box 30003, MSC 3Q, New Mexico State University, Las Cruces, NM 80003-8003 USA

Mapping of introgressed DNA from G. barbadense and G. hirsutum can improve genetic diversity and lead to dramatic genetic gain in fiber quality and other important traits in cultivated cotton. Interspecific-derived mapping populations (TM1 x NM24016 and NM24016 x 3-79) have been evaluated with RAPDs and SSRs. Linkage maps have been produced in both populations. Over 50 DNA markers have been anchored to individual cotton chromosomes. The majority of the anchored markers are in the form of flourescent labelled SSRs providing high-throughput potential for mapping. Large numbers of multiplex bins have been produced for this effort. QTLs in both populations have been identified for fiber strength, fiber length, and fiber fineness. The QTLs identified can be used to introgressive novel QTLs into cultivated cotton. The interspecific-derived populations were also used to create graphical genotypes of the progeny and the introgressed parent, NM24016, since much of the polymorphism can be assigned to chromatin from G. barbadense and G. hirsutum.