IDENTIFICATION OF AFLP AND SSR MARKERS LINKED CLOSELY TO NODULATION GENES IN SOYBEAN

¹ The Department of Biochemistry, The University of Queensland, Australia

² Plant Molecular Genetics and Center for Legume Research, The University of Tennessee, USA

Identification of AFLP and SSR markers linked closely to nodulation genes in soybean Searle, I. R.^1,2, Southerton, S. G. ¹, Gresshoff, P. M. ³ and Carroll, B. J ^1,2. ¹ Department of Biochemistry and ² School of Land and Food, The University of Queensland, Brisbane 4072, Australia. ³ Plant Molecular Genetics and Center for Legume Research, The University of Tennessee, Knoxville, Tennessee, U.S.A. Soybean genes have been identified that condition non-nodulation (nod49 and nod139) and supernodulation (e.g. nts382) ^{(1, 2)}. Three large F₂ populations were generated by crossing G. max L. (Merr.) cv. 'Bragg' (carrying mutant alleles of nod49, nod139 or nts382) to its wild relative G. soja (PI468.397). Using bulked segregant analysis, 3200 polymorphic AFLP markers were mapped relative to each locus, and 3, 15 and 20 AFLP markers were identified to be linked to nod49, nod139 and nts382 , respectively. SSR markers flanking nod 49 and nod 139 have also been identified. The long term aim of this project is to use closely linked markers to clone nod49, nod139 and nts 82. ¹ Mathews, A. et al.(1989) J.Hered. 80, 357-360. ² Carroll, B.J. et al. (1985) PNAS 82, 4162-4166.