1 Dept. of Agronomy & Range Science, University of California, Davis, One Shields Avenue, Davis CA 95616-8515 USA 2 Clemson University, Genomics Institute, Clemson University, Clemson, SC 29634 USA
A genomic BAC library of the A genome of wheat has been constructed. Triticum monococcum accession DV92 was selected for this purpose because it is a cultivated diploid wheat and one of the parental lines used in the construction of the highly saturated RFLP map of T. monococcum (Dubcovsky et al, 1996; Genetics 143: 983-999). Leaves from this accession were used to isolate high molecular DNA from nuclei. This DNA was partially digested with restriction enzyme Hind III, subjected to double size selection by PFGE, electroeluted and cloned into the pI451 IndigoBAC vector. Currently, the library has 92,160 clones sorted in 240 384-well plates with an average insert size of 120 kb. From those, 73,728 clones were gridded into 4 high density filters (18,432 clones each). The actual coverage of the library was estimated to be two genomes equivalents based on a total genome size of 5.6x109 bp. A four-genome equivalent coverage is expected in the near future. Three different well-spaced wheat chloroplast genes, and single copy RFLP markers are being used to characterize the library. This library will be an important tool for physical mapping in wheat and positional cloning of important agronomic genes.
Plant & Animal Genome VII Conference Plant & Animal Genome VII Conference