Plant & Animal Genome VI Conference Plant & Animal Genome VI Conference
W77
Kazusa DNA Research Institute, 1532-3 Yana, Kisarazu, Chiba 292, Japan
The Arabidopsis genome project at Kazusa DNA Research Institute
consists of three sub-projects: 1) large scale genome sequencing, 2)
cDNA sequencing, and 3) analysis of mutant lines by T-DNA-mediated gene
disruption. The genome sequencing project is being coordinated with other
members of Arabidopsis Genome Initiative. We are sequencing the long arm
and portions of the short arm of chromosome V, and the long arm of
chromosome III at an approximate rate of 700 kb per month. The finished
sequences are subjected to similarity search and computer-aided analysis
for prediction of coding regions. The annotated sequences are released on
the public DNA databases and on our web database, KAOS
[http://www.kazusa.or.jp/arabi/].
As of Nov. 1st, 1997, approximately 3.86 Mb sequences of chromosome V have
been released. For precise localization of both 5' and 3' ends of genes deduced
by genome sequencing, end sequencing of cDNA clones is carried out using cDNA
libraries rich in full-length molecules from various sources, which were
constructed either through normalization process or by concentration of
long cDNAs (>3kb) by size-fractionation.