Plant & Animal Genome VI Conference

P57

THE USE OF MICROSATELLITE MARKERS FOR BARLEY GENOTYPE CHARACTERIZATION

Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben

A barley Lambda phage library was screened with (GA)_n and (GT)_n probes for developing microsatellite markers. The number of repeats ranged for GA from 2-58 and for GT from 2-24. 15 selected microsatellite markers were polymorphic for barley. A total of 130 alleles were detected by 15 barley microsatellite markers. The number of alleles per microsatellite markers varied from 5-15. On average 8.6 alleles per locus were observed. These microsatellite markers were used to estimate the genetic diversity among 163 barley genotypes of Gene bank, Germany. Except the GMS4 all other barley microsatellite markers showed a high value of gene diversity ranged from 0.64-0.88. The mean value of gene diversity was in Wild type group 0.74, in Land races also 0.74 and even among the cultivars ranged gene diversity from 0.35- 0.88 with a mean of 0.72. There was no significant differences in polymorphism detected by GA and GT microsatellite markers. The estimated genetic distances revealed by microsatelllite markers were on average among the Wildtypes 0.75, Land races 0.72 and among cultivars 0.70. The microsatellite markers could distiguish between different barley genotypes. The high degree of polymorphisms of microsatellite markers allows a rapid efficient identification of barley genotypes.