PAG-VI: AFLP MARKERS

PAG-VI  Plant & Animal Genome VI Conference

Town & Country Hotel, San Diego, CA, January 18-22, 1998.


P87

AFLP MARKERS

Manuela Boritzki, Joerg Plieske, DARUSH STRUSS

    Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany

The high degree of polymorphism of AFLPs provide a efficient system for identification and genome analysis of sweet cherry cultivars. The cultivars of sweet cherry have been usually characterized by assessment of phenotypic and pomological traits. AFLP markers have been employed to identify 142 sweet cherry accessions and estimation the genetic diversity among this material. 10 of 18 tested primer combinations were informative with up to 80 bands per primer combination, 7-33% of the amplified bands were polymorphic depends on primer combination. A total of 142 polymorphisms were revealed in the 142 sweet cherry cultivars. All of the cultivar tested could be clearly identified. The cluster analysis revealed two distinct group of cultivars. Some cultivars showed a high degree on similarity. They might be clones of the same origin or very closed related accessions. AFLPs provide a means for identification and effective maintenance and utilization of sweet cherry genetic diversity.


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