Plant & Animal Genome VI Conference Plant & Animal Genome VI Conference
W73
Department of Soil & Crop Sciences, Texas A&M University, College Station, TX 77843-2474
Fluorescent in situ hybridization (FISH) of DNA to chromosomes
is a powerful tool for molecular cytogenetic analysis of plants.
FISH can be used to perform a variety of tasks that includes
assigning repetitive and single copy DNA sequences to positions
on chromosomes, assigning RFLP recombinational linkage groups
to specific chromosomes and chromosome arms, determining
relationships between recombination based and physical map
distances, detection of alien DNA in introgressed lines, and
detection of the number and sites of transgene inserts. FISH
of RFLP-selected BACs can provide anchor sites for walking to
genes of interest. Progress in molecular cytogenetics of cotton
will be discussed.