Plant & Animal Genome VI Conference Plant & Animal Genome VI Conference
W16
Departments of Zoology, and Genetics and Cell Biology, Washington State University, Pullman WA 99164-4236 USA
Rainbow trout are an important aquaculture species around the world which
are native to the Pacific coast of North America. Our laboratory has
developed five homozygous clonal lines in this species using the chromosome
set manipulation techniques of androgenesis and gynogenesis. Four lines
are YY (all-male) in genetic constitution and one is XX (all-female). Some
lines have distinctive attributes which should be of interest for genetic
analysis. The lines can be stored in the form of cryopreserved sperm for
transport and long-term storage. A genetic map has been developed based on
analysis of the doubled haploid progeny produced by androgenesis from the
hybrid between two of the lines (OSU and Arlee). This map is primarily
based on AFLP markers and demonstrates several notable features, including
AFLP clustering, linkage of one group of markers to sex, and apparent
tetrasomic inheritance of some markers. Future investigations utilizing
the clonal lines could include detailed genetic analysis of the sex
chromosomes, QTL analysis of distinctive traits, and their use in cancer
and immunology research.