Plant & Animal Genome VI Conference

P59

AN INTEGRATED SSR/RFLP/RAPD MOLECULAR GENETIC LINKAGE MAP OF SOYBEAN

1. USDA-ARS, Soybean and Alfalfa Research Lab, Bldg. 006, Rm. 100, BARC-West, Beltsville, MD 20705.
2. Department of Biology, University of Utah, Salt Lake City, UT 84112.
3. USDA-ARS-CICG, Department of Agronomy, Iowa State University, Ames, IA 50011.
4. BioGenetic Services Inc., 2308 6th St. E., P.O. Box 710, Brookings, SD 57006.
5. USDA-ARS, Soil Drainage Unit, 590 Woody Hayes Drive, Columbus, OH 43210.
6. Department of Horticulture and Crop Science, The Ohio State University and the Ohio Agric. Res. and Dev. Center, Wooster, OH 44691.
7. Department of Agronomy, University of Nebraska, Lincoln, NE 68583.

A total of more than 500 new Simple Sequence Repeat (SSR) DNA markers were developed in soybean. The SSRs along with RFLP, RAPD, classical, and a small number of AFLP markers were mapped in three soybean mapping populations: the USDA/Iowa State Glycine max x G. soja population, the University of Utah Minsoy x Noir 1 Recombinant Inbred Line population, and the University of Nebraska Clark x Harosoy population. The primary objectives of this research were to define a consensus linkage map of soybean that contained 20 linkage groups and to provide a large number of highly polymorphic "landmark markers" that would permit the easy identification of homologous linkage groups in other soybean molecular genetic maps and in QTL mapping studies. The development and mapping of more than 500, mostly trinucleotide, SSR markers was accomplished. Because three mapping populations were employed, it was possible to coalesce small linkage groups in one map by homology with larger groups in another. The resulting map now has the anticipated 20 linkage groups.