Plant & Animal Genome VI Conference Plant & Animal Genome VI Conference
W96
Recent advances in genomic molecular methods have dramatically
accelerated the rate of genomic research. Most of these advances involve
methods for the manipulation and analysis of DNA, and offer great
opportunities to further the scope of the field. While much attention
has been deservedly placed on these end processes, initial sample
preparation steps are also critical in ensuring downstream success. One
commonly overlooked aspect of genomic research has been DNA isolation.
Most genomic DNA isolations are done by traditional methods such as
phenol/chloroform extraction, CTAB/DTAB extraction, etc. These methods
are often very time and labor-intensive, and can involve numerous
technique-dependent manipulations. Elimination of these steps will be
necessary to maximize the potential of today's advanced molecular
techniques. Important factors for sample preparation success will be
presented. Sample type, quality and reliability, throughput, and total
cost should all be given consideration when selecting a sample
preparation method. An overview of commonly used sample preparation
methods will also be given, with emphasis on the advantages and
disadvantages of each to the genomic researcher. The latest advances
from QIAGEN will be introduced. These new systems should greatly
increase the speed and quality at which genomic sample preps are
processed. New 96-well systems allow throughput levels previously
unachievable due to personnel limits, and open the door to automated
solutions. Specialized plant systems for both DNA and RNA isolation
offer high-quality nucleic acid free of plant contaminants, in a
fraction of the time of traditional plant protocols. Advances in PCR
increase the stability and robustness of amplification systems. In
addition, researchers will have the opportunity to provide feedback on
their needs and concerns. This feedback will help QIAGEN continue
providing solutions to the genomic research community.