Plant & Animal Genome VI Conference Plant & Animal Genome VI Conference
W26
Laboratoire de Génétique Biochimique et de Cytogénétique, INRA, Centre de Recheches de Jouy, 78350 Jouy-en-Josas, FRANCE.
Compared with other domestic species, mapping of the horse genome is in its
early stage. The international effort to construct a low density genetic
map is well under way with the exchange, typing and linkage analysis of
reference families. In addition to this map, there is a need for physical
mapping to obtain an integrated map in a short period of time. BAC
libraries are considered to be a powerful tool to reach this objective. A
horse BAC library of about 40 000 clones was thus constructed and
characterized. The inserts have a mean size of 110kb representing a
coverage of 1.5 genome equivalent and the probability of finding a single
sequence is about 75%. The library was screened with type I primers
designed from sequences retrieved from international databases and type II
primers from plasmid-derived microsatellites produced in our laboratory.
The corresponding inserts were cytogenetically localized by FISH using R
banding. Results were consistent with published human-horse Zoo-FISH and
allowed the assignation and/or orientation of some known linkage groups and
the localization of a few genes.