Plant & Animal Genome VI Conference

P70

MICROSATELLITE POLYMORPHISM AMONG AND WITHIN BROILER CHICKEN LINES

1. 201 Kildee Hall, Department of Animal Science , Iowa State University, Ames, IA USA 50011
2. Avian Disease and Oncology Lab, USDA/ARS, 3606 East Mount Hope Road, East Lansing, MI USA 48823
3. Faculty of Agriculture, Hebrew University of Jerusalem, POB 12, Rehovot 76100, Israel

Two independent broiler chicken lines were genotyped with microsatellite markers to determine genetic polymorphisms both within and among broiler populations. Line L was a broiler breeder male line. Line C was derived from a commercial broiler line by divergent selection for antibody response, and the typed individuals represented F₁ progeny of high and low subline parents. Six individuals per line were genotyped with primers from the Poultry Genome Mapping Kits 1 and 2. These 245 mostly CA-repeat microsatellites were selected based upon genomic coverage and polymorphism on the East Lansing mapping reference population. The 59 primer sets chosen for current use provided wide genomic coverage. All primers amplified a PCR product in Line L, but two produced no product in Line C. Only one primer pair yielded monomorphic product in both lines. Of the primers generating product, there were 91.5 and 89.5% polymorphic primer pairs, respectively, for Line L and Line C. The average allele number per microsatellite was 2.8 and 2.9, respectively, for Line L and Line C. Considering the 57 primer pairs generating product in both lines, only 70 (27.7%) of 253 total alleles were shared between lines, with 44 primer pairs sharing alleles between lines. This study illustrates the highly polymorphic nature in broiler lines of microsatellites developed from Red Jungle Fowl or White Leghorn.