Plant & Animal Genome VI Conference Plant & Animal Genome VI Conference
P73
Dr.Peter DOVC, Department of Animal Science, Biotechnical Faculty, Groblje 3, SI-1230 Domzale, Slovenia
Two divergently selected chicken lines for high (D+) and low (D-) body weight at eight weeks of age were used as a source of chicken genomic DNA. After 19 generations of phenotypic selection mean body weight of chickens in D+ and D- line was 2524 g and 672 g, respectively. Both lines were derived from a common randombred base population. Genomic DNA from ten animals of each line (7 males and 3 females) was pooled and used for RAPD analysis. A set of 200 decamer oligonucleotides (UBC 501-600 and UBC 701-800) was screened for polymorphisms between both DNA pools. Three decamers (UBC-563, UBC-599 and UBC-788) revealed clear polymorphisms between pooled DNA samples. Primer UBC-599 turned out to be specific only for some animals from the pool but not for the entire phenotypic group. However, primers UBC-563 and UBC-788 gave group specific polymorphisms tested on 10 animals from each group. Primer UBC-563 amplified two group specific fragments (1000 bp and 600 bp), both present in all of ten D- animals but in none of D+ animals. The UBC-788 specific fragment (300 bp) was also present in D- but not in D+ animals. All three fragments were electroeluted from the gel, cloned and sequenced from both termini. The DNA sequences were compared with the sequences in the GenBank database and no significant sequence identity with any published sequence was found.