PAG-VI: COMPARATIVE MAPPING OF PIG HOMOLOGUES OF HUMAN GENES SHOWS COMPLETE SYNTENY CONSERVATION BETWEEN HSA3 AND SSC13 AND BETWEEN HSA13 AND SSC11

PAG-VI  Plant & Animal Genome VI Conference

Town & Country Hotel, San Diego, CA, January 18-22, 1998.

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COMPARATIVE MAPPING OF PIG HOMOLOGUES OF HUMAN GENES SHOWS COMPLETE SYNTENY CONSERVATION BETWEEN HSA3 AND SSC13 AND BETWEEN HSA13 AND SSC11

CHRISTOPHER KEITH TUGGLE1, Hsiao-Fang (Sunny) Sun1, Martine Yerle2, Patrick Chardon3, Max Frederick Rothschild1, Leslie Lyons4, Stephen O'Brien4

  1. Department of Animal Science, Iowa State University, Ames, IA 50011
  2. INRA- Laboratoire de Genetique Cellulaire, BP 27, 31326 Castanet-Tolosan, France
  3. Laboratoire de Radiobiologie Appliquee, INRA, 78352 Jouy en Josas Cedex, France
  4. Laboratory of Genomic Diversity, Bldg. 560, National Cancer Institute, Frederick, Maryland 21702-1201

A more detailed comparative map between the pig and human genome maps would allow identification of positional candidate genes for regions containing quantitative trait loci, and would also provide information for studies of chromosome evolution. To develop such comparative maps, we targeted two pig chromosomes, one containing a growth/carcass backfat QTL (chromosome 13, SSC13) and one where synteny comparisons have already been made among other species (chromosome 11, SSC11). Previous chromosome painting experiments indicated that human chromosome 3 (HSA3) represented much of SSC13, while human chromosome 13 (HSA13) painted SSC11. Eleven HSA3 and six HSA13 genes, spanning the entire human chromosomes, were selected and pig STSs were developed. Ten pig STSs homologous to HSA3 genes were regionally mapped using a somatic cell hybrid panel to SSC13 with 80-100% concordance. All six pig STSs homologous to HSA13 genes mapped to SSC11 with 85-100% concordance. Pig yeast artificial chromosome (YAC) and/or bacterial artificial chromosome (BAC) libraries were also screened with STS primers. Fluorescent In Situ Hybridization (FISH) mapping with identified YAC/BACs was used to further define gene order. These FISH data confirm painting results; however, the data also show extensive difference in gene order between HSA3 and SSC13. No clear gene order differences among examined loci on SSC11 and HSA13 were observed, in contrast to the different gene orders between HSA13 and bovine chromosome 12 (Sun et al., 1997). These data complement other comparative results and further demonstrate the extensive conservation of large syntenic groups between humans and pigs.

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