Plant & Animal Genome VI Conference

P7

RANDOM SEQUENCING ANLAYSIS OF RICE IMMATURE SEED ESTs FROM NORMAL AND SUBSTRACTED LIBRARIES

1. Dept. of Biological Science, Myong Ji University, Yongin, Korea
2. Dept. of Cytogentics, Natl. Inst. Agric. Sci. and Tech., Rural Development Adminstation, Suwon, Korea
3. Korea Basic Research Institute, Taejon, Korea
4. Dept of Agricultural Chemistry, Gyeongsang National University, Jinju, Korea
5. Dept of Agricultural Chemistry, Seoul National University, Suwon, Korea

The expressed sequence tags (ESTs) from rice immature seed, Oryza sativa cv Milyang 23, were partially sequenced and analyzed by BLAST homology search. About 5,000 cDNA clones were partially sequenced from two different cDNA libraries constructed with or without substraction of abundant mRNAs by hybridization with genomic DNA. About 20% of the ESTs were putatively identified as storage proteins such as glutelin, prolamine, albumin and globulin. And 10% of the clones were highly homologous to genes involved in metabolic pathways, particularly in starch biosynthesis and glycolysis. Compare to the ESTs with 30% of redundancy and putative identification in normal cDNA library, the only 15% of ESTs of the substracted library were putatively identified and redundant. The Korea Rice Genome Nework Server (http://bioserver.myongji.ac.kr) is maintained to provide the information of sequences, their homologies and alignments of the identified genes of the ESTs.