Plant & Animal Genome VI Conference

P12

ANALYSIS OF THE SUBTRACTED cDNA LIBRARIES OF THE CULTURED PREADIPOCYTES AND ADIPOCYTES.

Animal Genome Research Program Team, STAFF Institute, Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305, Japan

Meat quality is thought to be a highly complex trait. It contains several components such as fat content, tenderness, juiciness, and flavor. Many genes may influence these subcomponents. The intramuscular lipid is one of factors affecting the palatable quality of pork, especially tenderness. It has been said that the pork of Kagoshima (the south area in Kyusyu, Japan) Berkshire (KB) has advantages of meat quality compared to other breeds. The fatty acid composition of KB is higher in total saturated fatty acid and lower in mono- and polyunsaturated fatty acids than the fatty acid composition of other breed. To compare the difference of the gene expression involved in fatty acid composition, we constructed the subtracted cDNA libraries of the cultured porcine fibroblasts isolated from the back fat tissue of KB and Meishan x Goettingen (MG). We also constructed the subtracted cDNA libraries of the cultured adipocytes from KB and MG. The fibroblasts of KB and MG could be differentiated in culture media containing insulin (5 mg/ml), dexamethasone (1 mg/ml), octanate (1 mM), and clofibrate, lindeic acid, LY-171883, pantothenate, ascorbate ,100 mM, respectively. Several clones were obtained from these subtracted libraries. These clones had been subjected to sequencing to characterize the difference of genes expression between KB and MG.