Plant & Animal Genome VI Conference

C6

THE CONSTRUCTION OF THE FIRST Brassica GENOME DATABASE

1. Dept. Plant Breeding, Cornell University, Ithaca, NY, 14853
2. DNA LandMarks Inc., P.O. Box 6, Saint-Jean-sur-Richelieu, Quebec, Canada, J3B 6Z1
3. Dept. Plant Breeding, Cornell University, Ithaca, NY, 14853
4. USDA/ARS Plant Genetic Resources Unit, Cornell University, Geneva, NY

An ACEDB genome database for Brassica, called "CabbagePatch", was developed to give geneticists and breeders easy access to genome mapping data and DNA markers available in Brassica species. DNA LandMarks Inc., Quebec, Canada, sponsored the construction of the database and has contributed all the data currently contained in it. The data so far includes RFLP and PCR-based genetic maps of three Brassica species, B. juncea, B. napus and B. oleracea, which cover 3900 cM in 46 linkage groups and 15 smaller unassigned segments with over 800 molecular markers; the map positions of single genes such as the fertility restorer genes of Polima and Ogura-INRA CMS systems and quantitative trait loci for Blackleg and Clubroot disease resistance and seed glucosinolate are also presented. We have also included in the CabbagePatch information about 240 probes, mainly obtained from B. napus cDNA libraries. This is an ACEDB-type database, with hypertext links between classes and other web sites which provide an easy-to-use graphical interface. CabbagePatch can be accessed via the WWW at the National Agriculture Library at this URL: http://probe.nalusda.gov:8300/cgi-bin/browse/brassica.