Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W71 - UTILISATION OF MONO-ALLELIC MARKERS IN A MULTI-ALLELIC GERMPLASM
W71
UTILISATION OF MONO-ALLELIC MARKERS IN A MULTI-ALLELIC GERMPLASM
PELEMAN, JOHAN D.
Keygene n.v., Agro Business Park 90, P.O. Box 216, 6700 AE WAGENINGEN, The Netherlands
The utilisation of mono-allelic markers for indirect selection may pose problems when a broad predictive value within a complex germplasm is desired. This topic will be demonstrated by using the results obtained on the nematode resistance gene H1 in potato. In this study, it was clearly observed that many closely (< 2 cM) linked markers were very poor predictors for the presence of the H1 gene in the potato germplasm. No clear correlation could be observed between the degree of linkage to the H1 gene and the degree to which the respective marker could predict the presence of the H1 gene in the potato germplasm. This observation indicates that the poor predictive value of the markers can not be explained by the occurrence of recombination events which took place during the development of the germplasm varieties. Therefore we postulate that the poor predictive value of the markers is mainly caused by the presence of additional alleles in the germplasm which are (partly) also positively detected by some of the H1 linked markers. This postulate will be further confirmed with data obtained in corn. Solutions for obtaining markers with a broad predictive value will be discussed.