Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W68 - GENETIC ANALYSIS OF QUANTITATIVE AND QUALITATIVE TRAITS IN POTATO
W68
GENETIC ANALYSIS OF QUANTITATIVE AND QUALITATIVE TRAITS IN POTATO
GEBHARDT, CHRISTIANE(1), Ralph Schaefer-Pregl(1), Dario Leister(2), Agim Ballvora(3), Khalid Meksem(3), Francesco Salamini(3)
1. Max-Planck-Institut fuer Zuechtungsforschung, 50829 Cologne, Germany
2. The Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, UK
3. CNRS-Institut Des Sciences Vegetales, 91198 Gif sur Yvette Cedex, France
The aim of our work is the development of marker assisted selection schemes for potato breeding and the isolation and characterization of potato genes for resistance to pathogens. DNA marker technology was used to identify quantitative trait loci (QTL) for starch content in tubers. Genes functional in starch metabolism can be correlated by map position with QTL for starch content. Using RFLP markers, major loci for resistance to late blight, cyst nematodes and potato virus X have been mapped. High resolution maps were constructed for the R1 locus (late blight resistance) and the Gro1 locus (nematode resistance). By heterologous PCR using primers deduced from sequence motifs conserved between resistance genes of tobacco and Arabidopsis potato genomic DNA fragments were amplified that were homologous to resistance genes of other plants and cosegregated with the Gro1 gene. Positions on the potato map of other PCR derived fragments were correlated with positions of several resistance loci in syntenic maps of tomato and tobacco.