PAG-V: W64 - PHYSICAL MAPPING WITH RICE YACS ON THE HIGH-DENSITY GENETIC MAP

Plant & Animal Genome V Conference

Town & Country Hotel, San Diego, CA, January 12-16, 1997.

W64

PHYSICAL MAPPING WITH RICE YACS ON THE HIGH-DENSITY GENETIC MAP

UMEHARA, YOSUKE(1), Hiroshi Tanoue(2), Shoko Saji(2), Takanori Shimokawa(2), Kazuhiro Koike(2), Baltazar Alcaraz Antonio(2), Makiko Emoto(2), Atsuko Idonuma(2), Jianzhong Wu(2), Zi-Xuan Wang(2), Ikuo Ashikawa(2), Yuzo Minobe(3), Nori Kurata(3), Takashi Matsumoto(3), Takuji Sasaki(3)
1. Rice Genome Research Program, National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305, Japan
2. Rice Genome Research Program, STAFF Institute, Tsukuba, Ibaraki 305, Japan
3. National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305, Japan

We are conducting to construct a physical map of the rice genome with yeast artificial chromosome (YAC) clones using DNA markers on our high-density molecular genetic map. The rice YAC library (Umehara et al, 1995, Molecular Breeding 1: 79-89), which contains about 7,000 clones with average insert lengths of 350 kb and covers 6 times the rice genome length, was screened using mapped DNA markers reported earlier (Kurata et al, 1994, Nature Genetics 8: 365-372). With 1,285 of these markers, 2,648 YAC clones could be identified and 2,443 were assigned to unique locations on the chromosomes. The YAC clones aligned on the chromosomes formed 202 contigs, covering a total of 286 cM over the 12 rice linkage groups. These YAC contigs, together with the YAC clones outside contigs, are estimated to cover more than half of the rice genome. In the next step, we are focusing on chromosome 6 to examine what percentage of this chromosome can be covered with our YAC library. The results of these works will be presented.