Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W62 - FINE SCALE GENETIC AND PHYSICAL MAPPING OF A PHOTOPERIOD-SENSITIVITY GENE, <i>HD-1</i>, ON RICE CHROMOSOME 6.
W62
FINE SCALE GENETIC AND PHYSICAL MAPPING OF A PHOTOPERIOD-SENSITIVITY GENE, HD-1, ON RICE CHROMOSOME 6.
KATAYOSE, YUICHI(1), Yoshihide Kuboki(2), Yosuke Umehara(2), Lisa Monna(2), Utako Yamanouchi(2), Masahiro Yano(3), Takuji Sasaki(3)
1. Rice Genome Research Program, National Institute of Agrobiological Resources, Kannondai, Tsukuba, Ibaraki 305, Japan
2. Rice Genome Research Program, STAFF Institute, 466-1, Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305, Japan
3. Rice Genome Research Program, NIAR/STAFF, 1-2, Kannondai 2 Chome, Tsukuba, Ibaraki 305, Japan
Photoperiod-sensitivity is one of the factors associated with heading date in rice. We have already identified several QTLs controlling heading date using an F2 population derived from a cross between a japonica variety, Nipponbare, and an indica variety, Kasalath. One major QTL, Hd-1, was found to be involved in photoperiod-sensitivity. To isolate the gene at Hd-1, we have carried out fine scale linkage mapping using about 1500 segregating individuals and a pooled-sampling method. Simultaneously we identified 2 YAC clones harboring Hd-1. We have screened a cosmid library of Nipponbare with these YAC DNAs and with DNA clones which were mapped in the flanking region of Hd-1. As a result, 4 cosmid clones have been identified and found to overlap each other. In this way, we have isolated 100 kb genomic DNA sequences including Hd-1. Identification of expressed sequences located on the candidate genomic region is in progress. Complementation analysis by transformation of cosmid clones will also be necessary to conclusively identify Hd-1.