Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W51 - A NEW MAPPING POPULATION FOR HIGH RESOLUTION GENETIC MAPPING IN MAIZE
W51
A NEW MAPPING POPULATION FOR HIGH RESOLUTION GENETIC MAPPING IN MAIZE
LEE, MICHAEL
Room 1553 Agronomy Hall, Dept. of Agronomy, Iowa State University, Ames, IA 50011, USA
This presentation will summarize the status of a consortium's (ISU-Pioneer-DuPont) efforts to develop and characterize a set of inbred lines suitable for genetic mapping, cloning, and other genetic studies requiring improved genetic resolution. The lines were derived from a single-cross population of inbreds B73 and Mo17. Prior to line derivation, the F2 generation was randomly intermated for 4 generations. Subsequently, 370 F7:8 lines were produced by successive generations of self-pollination. The lines will be genotyped at RFLP loci at the maize 'bins' and anchor RFLP loci from other grasses and loci detected by PCR-based markers. Results of a pilot study, in which the map increased from 1520 cM and 11 linkage groups to 4564 cM and 28 linkage groups, will be presented. All information and materials related to this population will be available to the public. Seed of the inbreds will be available from ISU.