Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W49 - INRA MAIZE EXPRESSED GENOME MAPPING PROGRAM: STATE OF THE ART
W49
INRA MAIZE EXPRESSED GENOME MAPPING PROGRAM: STATE OF THE ART
CAUSSE, MATHILDE(1), Sylvain Santoni(2), Catherine Damerval(2), Janet Deatrick(2), Guy Decoux(2), Anne Marie Henry(2), Pascal Touzet(2), Alain Charcosset(2), Michel Zivy(2), Dominique de Vienne(2)
1. Station d'Amelioration des Plantes Maraicheres, INRA - Domaine Saint Maurice, BP 94 - 84143 - Montfavet Cedex (France)
2. Station de Genetique Vegetale, INRA/CNRS/UPS/INAPG, La Ferme du Moulon, 91190 Gif-sur-Yvette, France
The mapping effort in our laboratory has focused on the expressed maize genome. Three mapping populations (150 lines each) were derived from the 3 possible crosses between 3 distant lines. In addition the F2 population of one of these crosses and an independent doubled haploid population were used. With about 500 loci, the composite map covers 1900 cM (one marker every 3.8 cM). Marker loci are of two types: 382 RFLP markers of various origins (anonymous core markers, ESTs and known function genes or cDNA), 8 isozyme loci, and about 100 markers revealed by two dimensional electrophoresis of proteins of three organs (coleoptile, leaf and grain). In order to characterize the marker proteins, partial sequences of internal fragments of 43 proteins excised from the gels were determined, which allowed 22 of them to be identified. Individual and composite maps are used in various programs: characterizing QTL for drought stress, for grain development, marker-assisted selection, etc. A local database has been constructed to manage all theses data. As the data are being published, they become available on the www server (http:moulon.inra.fr/imgd), which will be presented.