Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W47 - A SYSTEM FOR PHYSICAL MAPPING OF MAIZE CHROMOSOMES
W47
A SYSTEM FOR PHYSICAL MAPPING OF MAIZE CHROMOSOMES
ANANIEV, EVGUENI V.(1), Oscar Riera-Lizarazu(1), Howard W. Rines(2), Ronald L. Phillips(3)
1. University of Minnesota, 503 Borlaug Hall, 1991 Buford Cr., St.Paul MN 55108
2. University of Minnesota, 504 Borlaug Hall, 1991 Buford Cr., St.Paul MN 55108
3. University of Minnesota, 516 Borlaug Hall, 1991 Buford Cr., St.Paul MN 55108
Maize (Zea mays L.) chromosome addition lines of oat (Avena sativa L.) recovered from oat x maize crosses are in effect a mean of cloning of individual maize chromosomes with oat serving as a host organism. The chromosome addition lines will be irradiated to produce oat lines with segments of individual maize chromosomes introgressed into the oat chromosomes. A collection of overlapping segments will be generated for each maize chromosome. Oat-maize introgression lines will serve as a source of DNA to construct genomic libraries of DNA fragments in an appropriate vector system such as cosmid, PAC, BAC or YAC. Clones with maize genomic DNA will be identified with the help of a multiprobe composed of a mixture of maize-specific, relative to oat, dispersed repetitive DNA sequences. Eventually contigs of cloned overlapping fragments -a physical map- will be constructed for each maize chromosome. Chromosome-specific arrayed libraries of cloned DNA fragments will be used for basic and applied research on maize genome organization. The first steps in realization of this project will be presented and discussed.