Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W44 - A CONSERVED REPETITIVE DNA ELEMENT LOCATED IN THE CENTROMERES OF CEREAL CHROMOSOMES
W44
A CONSERVED REPETITIVE DNA ELEMENT LOCATED IN THE CENTROMERES OF CEREAL CHROMOSOMES
JIANG, JIMING(1), Shuhei Nasuda(2), Fenggao Dong(3), Christopher W. Scherrer(4), Sung-Sick Woo(5), Rod A. Wing(6), Bikram S. Gill(7), David C. Ward(8)
312. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, U.S.A
313. Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, U.S.A
314. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA
315. Biotechology Center, University of Wisconsin-Madison, Madison, WI 53706, USA
316. Department of Soil and Plant Sciences, Texas A & M University, College Station, TX 77843, USA
317. Department of Agronomy and Biological Sciences, Clemson University, Clemson, SC 29634, USA
318. Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
319. Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, USA
A 745 bp repetitive DNA clone, pSau3A9, was isolated from sorghum (Sorghum bicolor). This DNA element is located in the centromeric regions of all sorghum chromosomes as demonstrated by fluorescence in situ hybridization. Repetitive DNA sequences homoeologous to pSau3A9 also are present in the centromeric regions of chromosomes from other cereal species, including rice, maize, wheat, barley, rye, and oats. Probe pSau3A9 also hybridized to the centromeric region of B chromosomes from rye and maize. The repetitive nature and its conservation in distantly related plant species indicate that the pSau3A9 family may be associated with centromere function of cereal chromosomes.