Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W35 - TRANSFORMATION OF LOBLOLLY PINE USING <i>Agrobacterium</i>
W35
TRANSFORMATION OF LOBLOLLY PINE USING Agrobacterium
GOULD, JEAN H.(1), Yuan-Xiang Zhou(2), Veera Padmanbahn(3), Maria Magallanes-Cedeno(4), Ronald J Newton(4)
1. Forest Science Department, Texas A&M University, College Station TX, 77843-2135
2. Forest Science Department, Texas A&M University, College Station TX, 77843
3. Horticultural Sciences Department, Texas A&M University, College Station TX 77843
4. Forest Science Department, Texas A & M University, College Station TX, 77843
We have used a shoot apex/Agrobacterium inoculation procedure to transform loblolly pine. The method is genotype-independent and yields shoots from which plants are recovered. The method was first applied to loblolly pine because of the improtance of this genotype in US commerce and because it was the most difficult pine for plant regeneration following transformation. The method used relies on the developmental drive in the shoot apex for plant regeneration and promotion of virulence in the biological vector for gene transfer. We have recovered plants exhibiting differential expression of the transferred beta-glucuronidase (GUS) gene, and carry both transferred GUS and NPTII genes in high molecular weight DNA, characteristic of genomic incorporation.