Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W31 - CHARACTERIZATION, GENETIC AND PHYSICAL MAPPING ANALYSIS OF 37 HORSE PLASMID AND COSMID-DERIVED MICROSATELLITES
W31
CHARACTERIZATION, GENETIC AND PHYSICAL MAPPING ANALYSIS OF 37 HORSE PLASMID AND COSMID-DERIVED MICROSATELLITES
Godard, Sophie(1), Daniel Vaiman(1), Anne Oustry(1), Milchele Nocart(1), Maud Bertaud(1), Stephane Guzylack(1), Jean-Claude Meriaux(2), Edmond Paul Cribiu(2), GERARD GUERIN(2)
1. INRA, Laboratoire de Genetique biochimique et de Cytogenetique, 78350, Jouy-en-Josas, France
2. Labogena , Domaine de Vilvert, 78350, Jouy-en-Josas, France
Thirty-seven new horse microsatellites (11 from plasmid libraries and 25 from a cosmid library) were isolated and characterized on a panel of four horse breeds. Thirty-one were found polymorphic with heterozygosities comprised between 0.12 and 0.88. Twenty-five of these new DNA markers were physically mapped to R-banded horse chromosomes 1, 3, 4, 9, 11, 12, 13 ,18, 19, 21, 22 and 23. Furthermore, linkage analysis between a selection of 42 DNA markers including those presented in this study, and 16 conventional markers of the horse hemotype was performed on 6 paternal halfsib horse families. Five linkage groups were detected, of which 3 were assigned to chromosomes 11 and 18, and two other linkages were highly suspected. Our cosmids label 13 out of the 31 horse autosomes. Moreover, the physical anchoring of part of these markers will help to assign and orientate linkage and synteny groups on the chromosomes.