Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: W18 - <i>Arabidopsis</i> GENOME SEQUENCING AT TIGR - CURRENT STATUS AND GOALS FOR THE FUTURE.
W18
Arabidopsis GENOME SEQUENCING AT TIGR - CURRENT STATUS AND GOALS FOR THE FUTURE.
ROUNSLEY, STEVE
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD USA 20850
In Fall of 1996, three US groups were funded to begin genomic sequencing of Arabidopsis. As one of the funded groups, TIGR has initiated sequencing regions of chromosome II, as part of the international Arabidopsis Genome Initiative. Coordination rather than competition is the key to efficient completion of the genome in a timely manner. Our general strategy has been to start with multiple points along the chromosome, and identify BAC clones from these starting regions by hybridization to YACs from the physical map of chromosome II. Shotgun sequencing is used on selected BAC clones to obtain high quality finished sequence of single clones from these regions. The finished sequence is annotated and submitted to the public sequence databases. We are also using BAC end-sequencing to identify BACs that overlap with the clones that have been sequenced, allowing us to extend our sequencing walk out in both directions from each starting point. The latest status and future goals for our sequencing project will be presented.