Plant & Animal Genome V Conference
Town & Country Hotel, San Diego, CA, January 12-16, 1997.
PAG-V: S3 - SINGLE MOLECULE APPROACHES TO GENOMIC ANALYSIS
S3
SINGLE MOLECULE APPROACHES TO GENOMIC ANALYSIS
Anantharaman, Thomas, Virginia Clarke, Steven Damo, Stephanie Delobette, Eileen Dimalanta, Joanne Edington, Joseph Giacalone,
Catharina Hiort, Xinghua Hu, Edward Huff, Benjamin Irvin, Junping Jing, John Lai, Ernest Lee, Bhubaneswar Mishra, Janaswami R. Murti,
Brett Porter, Rong Qi, Rodric Rabbah, Arvind Ramanathan, Jason Reed, Akhtar Samad, Alexander Shenker, Yiannis Skiadas, Khayama
Tankhoyeva, Weining Wang, Estarose Wolfson, DAVID C. SCHWARTZ
Department of Chemistry, New York University, 31 Washington Place, New York, NY USA 10003
Our laboratory has developed a new physical approach to genomic analysis named Optical Mapping, which uses modified surfaces to
fix DNA molecules for biochemical modification. Restriction enzymes are used to cleave fixed molecules, and after staining with a
non-specific fluorochrome, DNA fragments are analyzed under a microscope to produce high resolution, ordered restriction maps. The use of
sophisticated machine vision techniques and Bayesian modeling approaches enables fully automatic construction using an assortment of
cloning systems. Throughput is further enhanced by using large a number of samples arrayed by a robotic system on Optical Mapping surfaces,
and successful implementation of a fully integrated automated image collection/analysis system, featuring specialized file management and
distributive processing.