PAG-V: S22 - FISH PHYSICAL MAPPING IN PLANT SPECIES WITH LARGE GENOMES

Plant & Animal Genome V Conference

Town & Country Hotel, San Diego, CA, January 12-16, 1997.

S22

FISH PHYSICAL MAPPING IN PLANT SPECIES WITH LARGE GENOMES

LAPITAN, NORA L.V.(1), Susan E. Brown(2), Janice L. Stephens(2), Dennis L. Knudson(2)
1. Department Of Soil And Crop Sciences, Colorado State University, Fort Collins, CO
2. Department Of Bioagricultural Sciences And Pest Management, Colorado State University, Fort Collins, CO

While the idea of map-based cloning was novel ten years ago, it is now becoming commonplace for cloning disease genes in humans and important genes in Arabidopsis and tomato. At present, however, there are still doubts as to the practicality of map-based cloning in plant species with large and complex genomes. The current limitations in the genomics of these species include a lack of knowledge of the correspondence between the genetic and physical maps and low levels of polymorphism in some species, including barley and wheat. These are important because map-based cloning strategies depend on knowledge of physical distances between markers linked to a target gene and ability to find markers tightly linked to a gene. The unique applications of fluorescence in situ hybridization (FISH) for addressing these problems and a strategy for constructing a physical map of barley genes will be presented.