PAG-IV Plant Genome IV Conference

Town & Country Conference Center, San Diego, CA, January, 1995.

W47
Detection of Molecular Markers Linked to Resistance to the Root-Knot Nematode Meloidogyne arenaria in Myrobolan Plum (Prunus cerasifer EHR)

E. DIRLEWANGER(1), A.C Lecouls(1,2), G. Salesses(1) and D. Esmenjaud(2)
1. INRA, Station de Recherches sur les Especes Fruitieres et la Vigne, Centre de Bordeaux, BP 81, 33883 Villenave d'Ornon cedex, France
2. INRA, Laboratoire de Biologie des Invertebres, BP 2078, 06606 Antibes Cedex, France

Meloidogyne arenaria is a common root-knot nematode (RKN) which can cause severe damage to Prunus crops under Mediterranean climatic conditions. The use of resistant rootstocks appears to be the best control against this pest. One source of RKN resistance is the myrobolan plum (P. cerasifera) which host suitability ranges from highly resistant (P.1079 and P.2175) to good host (P.2646 and P.16.5). The study of host suitability of segregating F1 progenies (P.2175 x P.2646 and P.2175 x P.16.5) and backcrosses of P.1079 indicated that resistance was conferred by a single dominant gene heterozygous in P.2175 and homozygous in P.1079. P.2646 and P.16.5 were recessive for this gene. The bulked segregant analysis (BSA) with RAPD was used to identify markers linked to the P.2175 resistance gene. For each cross, the DNA of 15 F1 hybrids of each phenotype (resistant/host) were pooled. A total of 380 10-mer PCR primers were screened for bands present in the resistant pools and absent in the susceptible ones. Three primers, which produce markers cosegregating with the resistance, were tested on each individual of the two F1 progenies. The RAPD markers were located at 13.1 cM from the gene locus, at one side of the linkage group and at 5 and 11.3 cM on the other side of the linkage group.

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