Plant Genome IV Conference Plant Genome IV Conference
AFLP markers have been used to construct a moderately dense genetic linkage map of peach. Separate maps were derived for dominant markers linked in coupling from the male and female parents,and co-dominant markers were used to identify homologous linkage groups between the two maps. We estimate that the combined maps cover about two-thirds of the peach genome, at average marker densities of one marker per four centimorgans for the complete set of markers, and one marker per ten centimorgans for the framework markers. The AFLP map has been completely integrated with a previously published map based on RAPD, isozyme, and morphological markers, allowing direct comparisons between these marker systems. AFLP markers had a 42-fold greater information content on a per-primer basis, and a 4-fold greater information content on a per-locus basis, than RAPD markers. The greater number of loci assayed per AFLP primer combination and the greater resolution of similarly sized amplification products account for the greater information content. The large number of AFLP markers allowed us to identify a putative translocation event in the parents of the mapping population.