Plant Genome IV Conference Plant Genome IV Conference
The Korea Rice Genome Research Program (KRGRP) started in September 1994. This is a nationwide project in which many researchers from universities and institutes are involved. The major research areas of KRGRP are rice genome mapping, map-based cloning, cDNA sequencing, molecular breeding in practice, transformation, and data-base construction. A recombinant inbred population was recently developed at NASTI/RDA. The population, derived from the cross between [basically] indica rice variety Milyang 23 and japonica variety Gihobyeo, consists of 164 F11 recombinant inbred lines developed by single seed descent. The population is especially attractive because of its excellent structure and size for fine mapping of target genes and QTL analysis, the immediate availability of pure lines, the high level of polymorphism, and agronomically valuable characteristics embodied in these varieties. Over 1,000 mapped RFLP and new, partially sequenced cDNA markers are being targeted for mapping over next few years, and looking forward to have increased saturation provided by AFLP's and microsatellite markers through international collaboration. The Est I-2 and two RFLP markers, RG 109 and RG 220, were linked tightly at the distance less than 1cM with the sd-1 gene on chromosome 1. Large insert libraries of rice were constructed; approximately 15,000 YACs and 6,000 BACs. We are currently using them to determine the relationship between genetic and physical distances in the region of chromosome 1 with a large population, and to initiate a chromosome walk to clone the sd-1 gene. Activities and results of other research areas will be introduced and discussed.