PAG-IV Plant Genome IV Conference

Town & Country Conference Center, San Diego, CA, January, 1995.


W13
Defining cDNA/Mutant Correspondence in Maize

GEORGIA L. YERK-DAVIS
Agricultural Research Service-USDA, Curtis Hall, University of Missouri, Columbia, MO 65211, USA

Maize is a model system for identifying potential cDNA/mutant pairs because: 1) A well defined, high density map containing a large number of cDNA markers is available, 2) More than eight hundred mutants have been located to chromosome or chromosome arm, 3) There is a defined set of RFLP markers (UMC Maize Core Marker Set) which has defined polymorphism information for standard inbred lines and even coverage of the maize genome. A defined strategy for mapping large numbers of maize mutants relative to RFLP markers, a strategy known as interval mapping, has been developed. This method allows rapid placement of mutants into 20 cM intervals where potential cDNA/mutant associations may be determined. Potential pairs identified for chromosome 1 will be discussed.


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