Plant Genome IV Conference Plant Genome IV Conference
DNA sequencing by hybridization (SBH) is based on defining a set of positive oligonucleotide probes in a DNA sample. We are developing super-chips as a way to score 10- to 14-mer probes efficiently and accurately. Super-chips consist of an array of oligonucleotide arrays spaced by hydrophobic strips. The first generation of super-chips has been manufactured by dispensing of pre-made oligonucleotides to the support. The process is 100-fold more effective in terms of speed, cost and performance than direct synthesis of oligonucleotides on the support. Long oligonucleotides (10- to 14-mer) are scored by ligating support-bound and labeled-unbound 5- to 7-mer probes using target DNA as templates (SBH format 3). Obtained full match-mismatch discriminations range from 10-50 fold providing a bases for accurate and low cost mutation screening in, or sequencing of, over 10,000 bases in a single reaction.