Plant Genome IV Conference Plant Genome IV Conference
In Arabidopsis thaliana the translation elongation factor EF1-alpha is
encoded by four genes. The DNA sequence of a 63 kb region containing 3
of these genes was determined. The sequenced segment contains at least
11 additional genes. No functional regroupment was observed. One of
the genes corresponds to the ascorbate peroxidase gene described
previously. The other genes correspond to sequenced ESTs from
Arabidopsis or Rice but do not show homology to proteins within the
databases screened.A comparison of genomic and cDNA sequences show a
rather compacted genome with a mosaic structure of genes and
intergenic areas. In most genes a 5' intron is observed. Two YAC
clones (EG22C6 and CICXC9) were selected and allowed us to map the
genes to the top of chromosome 1. The complete sequence of three cDNAs
have been obtained: one gene product shows significant similarity to
the E. coli hypothetical protein F402; the gene 6D01 shows a leucine
zipper motif following several basic amino acids; the 5' end of the
third cDNA encodes a probable protein disulfide isomerases. The
remaining orfs show BLASTn or x similarities to different gene
families. A 500 bp sequence containing a regulatory element
controlling the EF1-alpha genes expression was found to be duplicated
10 kb upstream.
This work is part of the ESSA biotech program.