Plant Genome IV Conference Plant Genome IV Conference
A rice mutant, Awa-odori having a recessive gene accelerated internode overgrowth (ao), showed an overgrowth phenotype of internode elongation which is usually repressed at the vegetative stage. Therefore, ao mutant has a potential biochemical interest to study the mechanism of internode elongation in rice. Elongating internodes of mutant segregants were excised and used as materials for cDNA library construction. A total of 404 clones were randomly picked from the cDNA library and partially sequenced. The homology of each sequence was searched by blastn e-mail server against Genbank DNA database. About 22% of sequences were identified as homolog of putative genes. Homologs of phenylalanine ammonia-lyase and stress induced hydrophobic protein were highly abundan t, whereas, in spite of greenish tissues, the frequency of homologs involve d in photosynthesis were very low in this library. We also identified some homologs of genes encoding membrane associated proteins, such as annexin s and ankyrine. These results indicate that use of cDNA library from ao mutant makes it possible to isolate unique genes of rice.