PAG-IV Plant Genome IV Conference

Town & Country Conference Center, San Diego, CA, January, 1995.

P31
The Diversity of Rice and the Origins of Polymorphism Detected at Sequence-Tagged Sites

Susan Constantino(1), Adoracion Resurreccion(1), Corazon Villareal(1), Julie Ann Robeniol(1), Evelyn Liwanag(1), Behzad Ghareyazie(1), Zeba Seraj(3), Bao-Rong Lu(2), Gurdev Khush(1) and JOHN BENNETT(1)
1. Division of Plant Breeding, Genetics and Biochemistry and
2. Genetic Resources Center, International Rice Research Institute, PO Box 933, 1000 Manila, Philippines
3. Department of Biochemistry, University of Dhaka, Dhaka 1000, Bangladesh

A set of 100 restriction fragment length polymorphism (RFLP) markers on the Cornell-IRRI genetic map of rice were converted into sequence-tagged sites by terminal DNA sequencing. Fifty of the markers were RG random genomic clones and the other fifty were RZ cDNA clones. Oligonucleotide pairs were synthesized for the amplication of the marker loci by the polymerase chain reaction (PCR). All primer pairs amplified marker loci in Oryza sativa and O. longistaminata. The frequencies of amplicon length > polymorphisms (ALPs) and PCR-based RFLPs were determined within O. sativa, within the 8 AA genome species, within the genus Oryza, and between Oryza and related genera such as Porteresia. The data provide an indication of the range of evolutionary diversity over which sequence-tagged sites consitiute useful markers.
Supported by RF and BMZ/GTZ.

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