PAG-IV Plant Genome IV Conference

Town & Country Conference Center, San Diego, CA, January, 1995.

P11
Construction of a Bacterial Artificial Chromosome Library in Apple

FRANK SUOZHAN CHENG, Norman F. Weeden, Susan K. Brown and Julie R. Kikkert
Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA

Apple is an important fruit crop and has relatively small genome size (769 Mbp/1C), which is smaller than the model crop -- tomato (953 Mbp/1C). A Bacterial Artificial Chromosome (BAC) Library is being constructed in order to isolate some important genes using map-based cloning techniques in apple. The DNA sample is collected from cultivar 'Liberty', which has several important genes including scab resistance. The preliminary results showed that an average insert size is 110 kb. Factors affecting BAC insert size in fruit crops will be discussed.

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