PAG-IV Plant Genome IV Conference

Town & Country Conference Center, San Diego, CA, January, 1995.

P1
The CSUH Maize cDNA Project: A Progress Report

Kalpana Karra, Amy Fitzgerald, Cheri Harkrader, Dan Herrera, Paresh Patel and CHRIS BAYSDORFER
Department of Biological Sciences, California State University, Hayward, CA 94542, USA

The objective of this project is to characterize and functionally identify large numbers of maize genes. We have previously reported on partial sequence data generated from 650 cDNAs isolated from a mature maize leaf cDNA library. Here we report on partial sequence data from a combined total of over 1000 cDNAs from this library. The overall identification rate for this library has increased slightly to 22% of unique cDNAs while the duplication rate remains constant at 10%. Of the 78% of cDNAs that have not been functionally identified, a relatively high number (currently 15%) show similarity to unidentified cDNAs from other plant or animal species. Comparisons with other plant cDNAs reveal a core set of close to 40% of our unique cDNAs that are similar to cDNAs from these other plant species. Comparisons with maize cDNAs from three other maize libraries reveals a smaller than expected set of common (constitutive) maize cDNAs. The latest project statistics and homology results will be reported.

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