Plant Genome III Conference Plant Genome III Conference
Comparative mapping of legume genomes will provide useful tools for identification and cloning of genes for valuable agronomic traits. A set of "anchor" probes would be a useful tool for uniting present legume maps and for the development of future maps. We have been testing a set of peanut cDNA probes for their ability to serve as anchor probes for comparative mapping in other legumes. A subset of probes was selected for their ability to give strong signals and simple banding patterns in peanut. Selected probes were then tested on a "garden" blots containing an array of DNA from other legumes (alfalfa, clover, soybean, pea). Many of the peanut cDNAs gave complex RFLP patterns in other legumes, reducing their usefulness for comparative mapping. A number of the, peanut probes were also mapped in soybean.