Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: A RFLP LINKAGE MAP OF CULTIVATED SUNFLOWER(HELIANTHUS ANNUUS
L.)
A RFLP LINKAGE MAP OF CULTIVATED SUNFLOWER(HELIANTHUS ANNUUS
L.).
Laurent Gentzbittel 1, Felicity Vear 2, Andre Berville 3 and Paul
Nicolas 4. 1-G.I.E. CARTISOL, 4 rue Ledru, 63038 Clermont-Ferrand
Cedex 01, France. 2-INRA, Amelioration des Plantes, Domaine de
Crouelle, 63039 Clermont-Ferrand, France. 3-INRA -ENSAM, 2 place
P. Viala, 34060 Montpellier, France. 4-Universite Clermont-II, 4
rue Ledru, 63038 Clermont-Ferrand, France.
A genetic linkage map for sunflower (H. annuus n = 17) has
been constructed, based on RFLP DNA markers, using 3 segregating
F2 and two backcrossed progenies. The consensus map, produced
with the JoinMap package, contains 139 marker loci (85 cDNA and 8
genomic probes) in 18 linkage groups, covering a total of 637
recombination units. Morpho-agronomic traits, such as fertility
restoration, "white stigmat" color or branching were also placed.
Six DNA libraries were the source of probes, consisting of one
PstI and one HindIII genoniic libraries, and four cDNA libraries
from etiolated plantlets, green leaves, stamens and ovaries.
Thirteen hundred thirty probes were screened for their ability to
detect polymorphism among a representative set of sunflower
inbred lines, leading to 180 mapped markers (18% of the initial
probes). In order to maximize polymorphism, and to produce a
putative consensus map of sunflower, five mapping populations of
150 individuals each were produced and subjected to linkage
analysis. The consensus map was computed by means of the JOINMAP
computer package (link LOD = 3.0, map LOD = 2.0). It represents
the 'best fit' and is clearly subject to the consistency of the
available data. Adding new markers could reduce the number of
deduced groups to 17 linkage groups, being consistent with the
haploid number of sunflower. The probe set and map will be used
to facilitate sunflower breeding. Additional aspects of the map
and the extent of coverage of the total genome will be discussed.
This work was financed with private funds from the G.I.E.
CARTISOL, and a grant from the "Ministere de l'Agriculture" of
France.
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