Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: MAPPING OF QUANTITATIVE TRAIT LOCI IN BC2F1 POPULATIONS OF
COMMON WHEAT X TRITICUM TAUSCHII
MAPPING OF QUANTITATIVE TRAIT LOCI IN BC2F1 POPULATIONS OF
COMMON WHEAT X TRITICUM TAUSCHII.
A. K. Fritz, T. S. Cox, B. S. Gill and R. G. Sears, Wheat
Genetics Resource Center, Kansas State University, Manhattan, KS
66506
Triticum tauschii (Coss.) Schmal., as the D genome donor of
common wheat (Triticum aestivum L.), has the potential to make
significant contributions to wheat's gene pool for quantitative,
as well as qualitative traits. Restriction fragment length
polymorphism (RFLP), random amplified polymorphic DNA (RAPD), and
isozyme analyses have been used to analyze the BC2F1 populations
of common wheat x T. tauschii. RFLP probes were selected based
on their position on the existing T. tauschii map. A high level
of polymorphism exists between the two species and introgression
of T. tauschii DNA into the wheat genome has been achieved. One
protein, 9 RAPD and 26 RFLP loci were scored on 152 BC2F1,
derived lines. The data indicates a high level of genetic
exchange between hexaploid wheat and T. tauschii for most
chromosomes. However, genetic exchange between chromosome 4D of
wheat and 4D of T. tauschii was severely restricted in our
populations. Quantitative trait loci (QTL) analysis was
performed via multiple regression analysis, as well as the
MAPMAKER-QTL and QTL-STAT computer programs. A locus having a
positive effect on kernel weight was found to reside on
chromosome 3D. The effect of the T. tauschii genetic material on
performance was also examined. Increasing levels of the genetic
contribution of T. tauschii were associated in a significant
manner with a general decrease in grain yield and increase in
protein content.
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