Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: MULTIPLE LOCUS RFLP DESIGNED BULK SEGREGANT ANALYSIS OF SOYBEAN
CYST NEMATODE RESISTANCE QTL
MULTIPLE LOCUS RFLP DESIGNED BULK SEGREGANT ANALYSIS OF SOYBEAN
CYST NEMATODE RESISTANCE QTL.
Arnaldo R. Ferreira 1, Kathy Clayton 2, David M. Webb 2, and Paul
Keim 1. 1 Department of Biological Science, Northern Arizona
University, Flagstaff, AZ 86011-5640. 2 Pioneer Hi-Bred Int.,
P.O. Box 1004, Johnston, IA 50131.
Soybean cyst nematodes (SCN) represents one of the most
devastating diseases affecting soybean production. Breeding for
SCN resistance is difficult and would be much more efficient if
makers tightly linked to resistance genes were available for
marker aided selection. RFLP markers, loosely linked to SCN
resistance QTLs (Webb et al. 1994), were used to select bum from
a segregating recombinant inbred population (PI437.654 X
BSR-101). These bulks were fixed for resistance/susceptibility
alleles using seven RFLP markers defining three genomic regions.
RAPD analysis on restriction digested templates revealed 115
genetic markers differing between resistant and susceptible bulks
were identified. Segregation analysis of these markers found
many of these markers unreliable or were not linked to resistance
QTL. However, two RAPD markers were found tightly linked to
resistance QTL. Several other RAPD markers were more loosely
linked to the resistance QTL. One marker resulted in the linkage
between two previously unlinked RFLP markers associated with SCN
resistance. Multiple-locus bulk segregant analysis using RFLP
markers is a useful method for obtaining tightly linked markers
where only loose marker QTL associations exist.
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