Plant Genome II Conference
Town & Country Conference Center, San Diego, CA, January, 1994.
PG-II: IDENTIFICATION OF RAPD MARKERS FOR SEVERAL HESSIAN FLY
RESISTANCE GENES TN WHEAT
IDENTIFICATION OF RAPD MARKERS FOR SEVERAL HESSIAN FLY
RESISTANCE GENES TN WHEAT
I. Dweikat, F. Patterson, R. Ratcliffe*, and H. Ohm; Departments
of Agronomy and 'Entomology and *USDA-ARS, Purdue University,
West Lafayette, IN 47907
We have developed germplasm lines for the identification of
DNA markers closely associated with genes for resistance in wheat
to Hessian fly (Mayetiola destructor). Our collection includes a
series of near-isogenic lines (NILs) each containing a single
resistance locus and all sharing the same genetic background of
cultivar Newton, susceptible to all known H. fly biotypes. Using
RAPD analysis, we have identified DNA polymorphisms between the
resistant and susceptible NILs involving 10 different H. fly
resistance loci, with more than one potential marker for several
of these genes. In a number of cases we have verified linkage to
the H. fly resistance locus using cosegregation analysis in large
(>120 plants) F2 populations. Of these 10 resistance loci, at
least 5 are located on chromosome 5A. In an effort to estimate
the degree of gene clustering, we are in the process of
determining their exact locations using a ditelosomic line for 5A
as well as determining the genetic map distances between
identified markers.
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